山羊F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)

概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed
    参阅全部 IgG + IgM + IgA 二抗
  • 靶标种属Mouse
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c). This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 最小交叉反应
    Human more details
  • 免疫原

    Mouse IgG, IgA and IgM whole molecules

  • 偶联物HRP

性能

应用

Our Abpromise guarantee covers the use of ab6006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/120000. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed 图像

  • Ab6006 was used at dilution 1/3000 with the primary antibody ab2861 in WB. See the review on ab2861.

Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)参考文献

This product has been referenced in:
  • Bassagañas S  et al. Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of a2ß1 integrin and E-cadherin function. PLoS One 9:e98595 (2014). WB . Read more (PubMed: 24878505) »
  • Patel P  et al. The endogenous production of hydrogen sulphide in intrauterine tissues. Reprod Biol Endocrinol 7:10 (2009). WB . Read more (PubMed: 19200371) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that ab6006 is suitable for use with antibodies ab119484, ab80039 and ab110304.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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Thank you for your inquiry.

I am happy to confirm that ab6006 is a suitable secondary antibody for these two primary antibodies:

ab80039 and ab110304.

ab6006 detects the heavy and light chains of mouse IgG and IgM and IgA....

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Thank you for your enquiry. The originator of this antibody conjugated at a 2:1 ratio. There could be 1 or 2 peroxidase per antibody molecule. Peroxidase molecule randomly bind to Lysines of the antibody. If there are lysines at the antigen binding sit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"