山羊F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed (ab5870)

概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 最小交叉反应
    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Mouse IgG whole molecule.

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/10000.
ELISA 1/60000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed (ab5870)参考文献

This product has been referenced in:
  • Ulasov IV  et al. Survivin-driven and fiber-modified oncolytic adenovirus exhibits potent antitumor activity in established intracranial glioma. Hum Gene Ther 18:589-602 (2007). WB . Read more (PubMed: 17630837) »

See 1 Publication for this product

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Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Pl...

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Thank you for your question. Both antibodies are only a F(ab)2 Fragment of antibody itself (therefore these antibody will not give F(c) mediated binding), but they different in the immunogens used and therefore epitopes recognized: Ab5887 was raised ag...

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Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I...

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Thank you for your clarification and for sharing more details with us. Certainly, we can offer you a replacement vial - free of charge. Would you be so kind to confirm your Purchase Order Number, or Abcam Order Number. We look forward to hearin...

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Thank you for getting back to us and providing more details of your protocol. We are very sorry to hear that you are not happy with our product. We have searched our database and found that this is a popular selling product and your feedback is the ...

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Thank you for getting back to us and providing more details of your protocol. We are very sorry to hear that you are not happy with our product. We have searched our database and found that this is a popular selling product and your feedback is the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"