ab98635 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Alkaline Phosphatase.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/1000 - 1/10000. as primary
1/1000 - 1/10000. by chemiluminescent detection
1/100 - 1/1000.
This product has been referenced in:
Wagner JT & Podrabsky JE Extreme tolerance and developmental buffering of UV-C induced DNA damage in embryos of the annual killifish Austrofundulus limnaeus. J Exp Zool A Ecol Genet Physiol323:10-30 (2015).
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