山羊F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed (ab5881)

概述

  • 产品名称
    山羊F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse or Human Serum Proteins.
  • 经测试应用
    适用于: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • 最小交叉反应

    Cow, Horse, Human more details
  • 免疫原

    Mouse IgG F(c) fragment

  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.424% Potassium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Goat F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed (ab5881)参考文献

This product has been referenced in:
  • Tjiam MC  et al. Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification. J Immunol 194:5320-8 (2015). Read more (PubMed: 25911748) »

See 1 Publication for this product

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