山羊F(ab')2抗小鼠IgG - (Fab)'2 (PE),预吸附二抗(ab5889)

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概述

  • 产品名称
    山羊F(ab')2抗小鼠IgG - (Fab)'2 (PE),预吸附二抗
    参阅全部 IgG 二抗
  • 宿主
    Goat
  • 靶标种属
    Mouse
  • 经测试应用
    适用于: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • 最小交叉反应

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
Immunomicroscopy Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

文献

This product has been referenced in:
  • Shi Q  et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). Read more (PubMed: 29096715) »
  • Carocci M  et al. Encephalomyocarditis Virus 2A Protein Is Required for Viral Pathogenesis and Inhibition of Apoptosis. J Virol 85:10741-54 (2011). Read more (PubMed: 21849462) »

See all 2 Publications for this product

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