山羊F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP)， pre-adsorbed (ab98762)

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概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP)， pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: WB, ELISA, IHC-P, ICCmore details
  • 最小交叉反应
    Human, Rat more details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/25000. by chemiluminescent detection. Use at 1/2000 - 1/20000 by colorimetric detection.
ELISA 1/10000 - 1/100000. as primary
IHC-P 1/200 - 1/2000.
ICC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed (ab98762)参考文献

ab98762 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab98762？请让我们知道，以便我们可以引用本数据表中的参考文章。

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4 Abreviews or Q&A

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Votes: Highest First

for example this secondary antibody: http://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fab-2-HRP-pre-adsorbed-ab98762.html
It is a full-length human protein with a C-terminally located Fc-tag.
We want to detect sialic...

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Thank you very much for the time taken giving me furthrt infotmation.

I can confirm that the Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (HRP), pre-adsorbed could overcome the cross-reactivity with the human Fc. However w...

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vielen Dank für Ihre Informationen!

Nun hat sich aber wieder etwas geändert, denn wir möchten jetzt Anti-Maus-Sekundärantikörper im F(ab)2 Format verwenden, nach wie vor HRP gekoppelt.

Haben Sie da etwas im Angebot, das Epitop sol...

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Vielen Dank für Ihre Anfrage.
Wir haben jetzt eine Bestätigung von Labor:
Wir haben einen Antikörper, der selbst ein F(ab)2 Fragment ist (gekoppelt an HRP und auch nur F(ab)2 Fragmente bindet:
ab98659 (Goat F(ab')2 polyclonal Secondary A...

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Thank you so much for your reply. Before I am going to purchase it may I get 5-10ul for test? That would be very helpful.

Thank you for your reply. We do not provide samples of our antibodies but under our Abpromise we do guarantee our products to work as stated on the datasheet. If they fail to perform as stated then we will happily replace or refund the antibody...

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I am looking for anti-mouse IgG heavy chain specific HRP for western blot. The size of my target protein is same with IgG light chain. I need avoid the light chain. I found several products on your website. Is that antibody to mouse IgG-Fc(HRP) can avo...

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Thank you for contacting Abcam. Based on your requirements, I would recommend ab20043 as it will only react with the heavy chain of the mouse IgG. The image below shows the different regions of the IgG molecule and so from that you will be able to see ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"