山羊F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed (ab5887)

概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • 最小交叉反应
    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/20000.
ELISA 1/100000. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed (ab5887)参考文献

ab5887 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Pl...

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Thank you for your question. Both antibodies are only a F(ab)2 Fragment of antibody itself (therefore these antibody will not give F(c) mediated binding), but they different in the immunogens used and therefore epitopes recognized: Ab5887 was raised ag...

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Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"