山羊F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886)

概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgGF(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • 最小交叉反应
    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/100000.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886)参考文献

ab5886 has not yet been referenced specifically in any publications.

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Although, all these three secondary antibodies (ab5886, ab98756, ab98657) recognize mouse IgG and were raised in goat, they are from different sources. The tested application/species/co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"