山羊F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed (ab7006)

概述

  • 产品名称山羊F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Human
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 最小交叉反应
    Cow, Horse, Mouse, Rat more details
  • 免疫原

    Full length native Human IgG (purified).

  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.60
    Preservative: 0.05% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 共轭说明R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.

Goat F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed (ab7006)参考文献

This product has been referenced in:
  • Alvarez-Cienfuegos A  et al. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Sci Rep 6:28643 (2016). Read more (PubMed: 27345490) »
  • Tiwari A  et al. Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds. Curr Protoc Stem Cell Biol 28:Unit 1C.15. (2014). Read more (PubMed: 24510768) »

See all 2 Publications for this product

Product Wall

Since there are more than one antibody attached to each phycoerythrin (PE) molecule, the antibody concentration is irrelevant in this case. It only takes one active antibody for a PE-antibody conjugate to bind to its antigen. Other antibody molecule...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"