山羊F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

概述

  • 产品名称
    山羊F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Human
  • 经测试应用
    适用于: Flow Cytmore details
  • 最小交叉反应

    Mouse, Rat more details
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明

    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/200.

Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)参考文献

ab98596 has not yet been referenced specifically in any publications.

Product Wall

Application
Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5ug/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.

PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.
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提交于 Apr 25 2017

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