山羊F(ab')2抗人IgG - Fc (PE),预吸附二抗(ab98596)
Key features and details
- Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗人IgG - Fc (PE),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Human -
经测试应用
适用于: Flow Cytmore details -
最小交叉反应
Mouse, Rat more details -
偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin. -
克隆
多克隆 -
同种型
IgG -
常规说明
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab98596于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | (1) |
1/50 - 1/200.
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说明 |
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Flow Cyt
1/50 - 1/200. |
图片
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Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10 μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5 min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5 µg/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.
PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (12)
ab98596 被引用在 12 文献中.
- Gao J et al. Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion. Phytomedicine 118:154942 (2023). PubMed: 37421767
- Wang Z et al. ACE2 can act as the secondary receptor in the FcγR-dependent ADE of SARS-CoV-2 infection. iScience 25:103720 (2022). PubMed: 35005526
- Kuo YC et al. Antibody-based redirection of universal Fabrack-CAR T cells selectively kill antigen bearing tumor cells. J Immunother Cancer 10:N/A (2022). PubMed: 35728874
- Okubo K et al. Inhibitory affinity modulation of FcγRIIA ligand binding by glycosphingolipids by inside-out signaling. Cell Rep 35:109142 (2021). PubMed: 34010642
- Mutoh T et al. Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis. Nat Commun 11:1253 (2020). PubMed: 32152303