山羊F(ab) Anti-Rabbit IgG H&L (HRP) (ab7171)

概述

  • 产品名称山羊F(ab) Anti-Rabbit IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 免疫原

    Rabbit IgG whole molecule

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • 共轭说明Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Goat F(ab) Anti-Rabbit IgG H&L (HRP) (ab7171)参考文献

This product has been referenced in:
  • West A  et al. Npas4 is activated by melatonin, and drives the clock gene Cry1 in the ovine pars tuberalis. Mol Endocrinol 27:979-89 (2013). Read more (PubMed: 23598442) »
  • Bayne RA  et al. Prostaglandin E2 as a regulator of germ cells during ovarian development. J Clin Endocrinol Metab 94:4053-60 (2009). Read more (PubMed: 19602557) »

See all 2 Publications for this product

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If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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