山羊F(ab) Anti-Mouse IgG H&L (FITC) (ab6669)

概述

  • 产品名称
    山羊F(ab) Anti-Mouse IgG H&L (FITC)
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • 免疫原

    Mouse IgG whole molecule

  • 偶联物
    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • 应用说明
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 文献

    This product has been referenced in:
    • Kochegarov A  et al. A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos. J Biomed Sci 20:20 (2013). Read more (PubMed: 23522091) »
    • Mogilenko DA  et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012). Read more (PubMed: 22271762) »

    See all 3 Publications for this product

    客户评价及客户问答

    Abreviews
    Application
    Flow Cytometry
    A431 cells were corrected using accutase.
    After incubation with an anti-EGFR antibody, these cells were stained with this anti-mouse IgG-FITC antibody for 30 min at RT.
    isotype control (Blue) was mouse IgG.
    anti-EGFR antibody (Red)
    Dilution: 1/1000 (1.0x10^6 cells/mL)
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    Verified customer

    提交于 Jul 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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