山羊F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33109)

概述

  • 产品名称山羊F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC)
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: WB, Electron Microscopy, ICC/IFmore details
  • 免疫原

    Mouse IgG whole molecule.

  • 偶联物FITC + Gold 1.4nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 0.1% BSA, 20mM PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Chromatographically purified through gel filtration columns.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明

     

    This product is a revolutionary new immunocytochemical reagent. This unique antibody probe contains a molecular label which contains both the 1.4nm ultra-small gold particle and fluorescein, enabling both fluorescence and electron microscope observation of a sample stained in a single labelling procedure. This probe is smaller than a whole IgG molecule, does not aggregate, and fluorescence quenching due to the goold particle is negligible. Normal methologies may be used successfully with this product. Due to some quenching of fluorescence by the gold particle, slightly higher concentrations of antibody are recommended for incubations. A blocking agent of 5% non-fat dried milk has been found to reduce background in some cases: this should be used before incubation with probe (in standard wash/blocking steps), and additionally, the Fluorescein-ultra-small gold probe should be diluted in a solution also containing 5% non-fat dried milk before it is applied.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33109)参考文献

ab33109 has not yet been referenced specifically in any publications.

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This is not very clear on the datasheet of ab33109, but this is actually a Fab' fragment conjugated to both ultra small ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"