By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine IgG and with light chains common to other bovine immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline phosphatase.