山羊抗鸡IgY H&L (HRP) (ab97135)

概述

  • 产品名称
    山羊抗鸡IgY H&L (HRP)
    参阅全部 IgY 二抗
  • 宿主
    Goat
  • 靶标种属
    Chicken
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • 经测试应用
    适用于: ICC, IHC-P, ELISA, WBmore details
  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.05% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明
    Molar enzyme/ antibody protein ratio is 4:1
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    Part of the AbExcel range.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

图片

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 90 seconds
  • Ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). Ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.

    This image is courtesy of an anonymous Abreview.

文献

This product has been referenced in:
  • Stefanova NA  et al. Association of cerebrovascular dysfunction with the development of Alzheimer's disease-like pathology in OXYS rats. BMC Genomics 19:75 (2018). Read more (PubMed: 29504901) »
  • Meunier M  et al. Promising new vaccine candidates against Campylobacter in broilers. PLoS One 12:e0188472 (2017). ELISA . Read more (PubMed: 29176789) »

See all 11 Publications for this product

客户评价及客户问答

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Abreviews
Application
Western blot
Antibody was diluted 1/2000 in TBST and incubated 1h at RT
Detection performed using ECL+
Primary antibody used was ab13989.
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提交于 Oct 01 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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