山羊抗鸡IgY H&L (HRP) (ab97135)
Key features and details
- Goat Anti-Chicken IgY H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
概述
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产品名称
山羊抗鸡IgY H&L (HRP)
参阅全部 IgY 二抗 -
宿主
Goat -
靶标种属
Chicken -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
经测试应用
适用于: ICC, IHC-P, ELISA, WBmore details -
偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
相关产品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97135于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent dilution.
|
|
IHC-P |
1/200 - 1/5000.
|
|
ELISA | (1) |
1/10000 - 1/100000. (Primary).
|
WB | (1) |
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000
|
说明 |
---|
ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (Primary). |
WB
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000 |
图片
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All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 90 seconds -
All lanes : Anti-Vimentin antibody (ab24525)
All lanes : Mouse cardiac fibroblasts
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab97135) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 seconds
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Ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). Ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.
This image is courtesy of an anonymous Abreview.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab97135 被引用在 36 文献中.
- Fazal SV et al. SARM1 detection in myelinating glia: sarm1/Sarm1 is dispensable for PNS and CNS myelination in zebrafish and mice. Front Cell Neurosci 17:1158388 (2023). PubMed: 37091921
- Kang SG et al. Innate Immune Status of Glia Modulates Prion Propagation in Early Stage of Infection. Cells 12:N/A (2023). PubMed: 37508542
- Enders L et al. Pharmacological perturbation of the phase-separating protein SMNDC1. Nat Commun 14:4504 (2023). PubMed: 37587144
- Hines MR et al. Extracellular biomolecular free radical formation during injury. Free Radic Biol Med 188:175-184 (2022). PubMed: 35724853
- Liu CW et al. Blood-Brain Barrier Disruption in Preclinical Mouse Models of Stroke Can Be an Experimental Artifact Caused by Craniectomy. eNeuro 9:N/A (2022). PubMed: 36224001