山羊Anti-Chicken IgY H&L (HRP) (ab6877)

概述

  • 产品名称
    山羊Anti-Chicken IgY H&L (HRP)
    参阅全部 IgY 二抗
  • 靶标种属
    Chicken
  • 经测试应用
    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    chicken IgY whole molecule

  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    Horseradish Peroxidase (HRP)
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/10000.
Dot blot Use at an assay dependent dilution.
ELISA 1/50000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/20000. PubMed: 16543490

图片

  • IHC image of beta Actin staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.
  • Ab6877 was used at dilution 1/200 with the primary antibody ab17517 in IHC-P. See the review on ab17517.

文献

This product has been referenced in:
  • Kim HJ & Han SJ Anodal Transcranial Direct Current Stimulation Provokes Neuroplasticity in Repetitive Mild Traumatic Brain Injury in Rats. Neural Plast 2017:1372946 (2017). Read more (PubMed: 28770112) »
  • Bobeck EA  et al. Oral antibodies to human intestinal alkaline phosphatase reduce dietary phytate phosphate bioavailability in the presence of dietary 1a-hydroxycholecalciferol. Poult Sci 95:570-80 (2016). Read more (PubMed: 26666254) »

See all 23 Publications for this product

客户评价及客户问答

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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