By immunoelectrophoresis and ELISA ab112772 reacts specifically with Duck, Sparrow, Dove and Chicken IgG. Turkey, Ostrich, Emu, Quail, Macaw and Cockateil IgG are also detected. No antibody was detected against non-immunoglobulin serum proteins. ab112772 may cross react with IgG from other species.
Goats were immunized with purified IgG from Dove, Duck, Sparrow and Chicken serum. ab112772 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).