山羊Anti-Armenian仓鼠IgG H&L (Biotin) (ab5744)

概述

  • 产品名称山羊Anti-Armenian仓鼠IgG H&L (Biotin)
    参阅全部 IgG 二抗
  • 靶标种属Armenian Hamster
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Cross reactivity against mouse has not been determined.
  • 经测试应用适用于: WB, ICC/IF, Dot blot, ELISA, Immunomicroscopy, IHC-Pmore details
  • 免疫原

    Armenian Hamster IgG whole molecule

  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000.
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ELISA 1/250000.
Immunomicroscopy Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Goat Anti-Armenian Hamster IgG H&L (Biotin) 图像

  • Ab5744 was used at dilution 1/500 with the primary antibody ab33483 in IHC-P. See the review on ab33483.

Goat Anti-Armenian Hamster IgG H&L (Biotin) (ab5744)参考文献

This product has been referenced in:
  • Accart N  et al. Revisiting fixation and embedding techniques for optimal detection of dendritic cell subsets in tissues. J Histochem Cytochem 62:661-71 (2014). Read more (PubMed: 24874853) »
  • Yu P  et al. Diet-induced obesity drives negative mouse vein graft wall remodeling. J Vasc Surg N/A:N/A (2013). Read more (PubMed: 23876511) »

See all 2 Publications for this product

Product Wall

The appropriate secondary antibody for use with this product will be a Secondary Antibody to Armenian Hamster IgG such as ab5744.

Thank you for contacting us. I am very glad to hear that you will be using our products in your experiments. I can gladly recommend three biotin labeled antibodies to suit your needs and have placed links for biotin labeled antibodies below. I would...

Read More

Thank you for this information.

I have noted that this primary/secondary antibody combination has been used and reviewed by another customer. Their Abreview includes a detailed protocol which you may wish to review. This review is at:http://...

Read More

Thank you for contacting us.

This product is not tested against or cross adsorbed against mouse. Since this is not a cross adsorbed secondary antibody, cross reactivity with other species may often be expected.

I have searched our...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"