Vector coding for a partial recombinant fusion protein, corresponding to amino acids 320-368 of Fruit fly (Drosophila melanogaster) GnRHR.
Target sequence used to make the antibody: GLYNIRGRMN NNNPSVNNRH TSLSNRLDSS NQLMQKQLTN NSLLNGRGQ.
All lanes : Anti-GnRHR antibody (ab52673) at 1/1000 dilution
Lane 1 : total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen Lane 2 : total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein).
Secondary All lanes : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution