概述

  • 产品名称Anti-GnRH抗体
    参阅全部 GnRH 一抗
  • 描述
    小鼠单克隆抗体to GnRH
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: QHWSYGLRPG GKRDAENLID SFQEIVKEVG QLAETQRFEC TTHQPRSPLR DLKGALESL IEEETGQKKI, corresponding to amino acids 24-93 of Human GnRH (NP_000816) with a tag (MW of the tag alone is 26 KDa).

  • 阳性对照
    • Recombinant tagged Human GnRH fragment.

性能

应用

Our Abpromise guarantee covers the use of ab76560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged GnRH is approximately 30ng/ml if used as a capture antibody.

靶标

  • 相关性Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • GnRH associated peptide 1 antibody
    • GNRH1 antibody
    • Gonadotrophin releasing hormone 1 antibody
    • GRH antibody
    • HH12 antibody
    • LHRH antibody
    • LNRH antibody
    • Luliberin I antibody
    • Luteinizing releasing hormone antibody
    • Lutenizing hormome releasing hormone antibody
    • Progonadoliberin 1 antibody
    • Progonadoliberin I antibody
    • Prolactin release inhibiting factor antibody
    see all

Anti-GnRH antibody 图像

  • Anti-GnRH antibody (ab76560) at 1 µg/ml + Recombinant tagged human GnRH fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 10 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

Anti-GnRH antibody (ab76560)参考文献

ab76560 has not yet been referenced specifically in any publications.

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