概述

  • 产品名称
  • 描述
    兔多克隆抗体to GnRH
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-FoFr, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Hamster, Pig, Xenopus laevis, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse GnRH aa 22-31. Modified residues - 22 with pyrrolidone carboxylic acid, 31 with glycine amide.
    Sequence:

    EHWSYGLRPG


    (Peptide available as ab41769)

  • 阳性对照
    • Mouse brain tissue sections.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯化说明
    Purified via ammonium sulfate precipitation.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/5000.

4% paraformaldehyde is recommended as a fixative.

ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22348098
IHC-Fr Use at an assay dependent concentration. PubMed: 19479999

靶标

  • 相关性
    Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • GnRH associated peptide 1 antibody
    • GNRH1 antibody
    • Gonadotrophin releasing hormone 1 antibody
    • GRH antibody
    • HH12 antibody
    • LHRH antibody
    • LNRH antibody
    • Luliberin I antibody
    • Luteinizing releasing hormone antibody
    • Lutenizing hormome releasing hormone antibody
    • Progonadoliberin 1 antibody
    • Progonadoliberin I antibody
    • Prolactin release inhibiting factor antibody
    see all

图片

  • Immunolocalization of GnRH in mouse embryo forebrain using ab5617 at a 1/1000 dilution.

文献

This product has been referenced in:
  • Soylu-Kucharz R  et al. Metabolic and behavioral effects of mutant huntingtin deletion in Sim1 neurons in the BACHD mouse model of Huntington's disease. Sci Rep 6:28322 (2016). IHC . Read more (PubMed: 27334347) »
  • Song B  et al. Effect of Aqueous Extract from Morinda officinalis F. C. How on Microwave-Induced Hypothalamic-Pituitary-Testis Axis Impairment in Male Sprague-Dawley Rats. Evid Based Complement Alternat Med 2015:360730 (2015). Read more (PubMed: 26435724) »

See all 10 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (Hypothalamus)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Hypothalamus
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kevin Zhang

Verified customer

提交于 Nov 17 2017

Application
IHC - Wholemount
Sample
Astatotilapia burtoni Tissue (Brain (CLARITY cleared with Passive clearing))
Specification
Brain (CLARITY cleared with Passive clearing)
Username

Sebastian Alvarado

Verified customer

提交于 May 02 2016

Thank you for contacting us. Both the ab5617 and ab16216 are raised against an immunogen synthetic peptide of HWSYGLRPG corresponding to amino acids 22-31 of GnRH. Comparing this to the corresponding sequence of Chicken GnRH (Swiss-Prot number ...

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Thank you for your enquiry. The concentration of the lot that received in November is approximately 1 mg/ml. Our current batch also has a concentration of approximately 1 mg/ml. Please contact us again if you have any additional questions.

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you dec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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