概述

  • 产品名称
  • 描述
    兔多克隆抗体to GnRH
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-FoFr, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Hamster, Pig, Xenopus laevis, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse GnRH aa 22-31. Modified residues - 22 with pyrrolidone carboxylic acid, 31 with glycine amide.
    Sequence:

    EHWSYGLRPG


    (Peptide available as ab41769)

  • 阳性对照
    • Mouse brain tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab5617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/5000.

4% paraformaldehyde is recommended as a fixative.

ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22348098
IHC-Fr Use at an assay dependent concentration. PubMed: 19479999

靶标

  • 相关性
    Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • GnRH associated peptide 1 antibody
    • GNRH1 antibody
    • Gonadotrophin releasing hormone 1 antibody
    • GRH antibody
    • HH12 antibody
    • LHRH antibody
    • LNRH antibody
    • Luliberin I antibody
    • Luteinizing releasing hormone antibody
    • Lutenizing hormome releasing hormone antibody
    • Progonadoliberin 1 antibody
    • Progonadoliberin I antibody
    • Prolactin release inhibiting factor antibody
    see all

图片

  • Immunolocalization of GnRH in mouse embryo forebrain using ab5617 at a 1/1000 dilution.

文献

This product has been referenced in:
  • Kim YJ  et al. WDR11-mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome. EMBO Rep 19:269-289 (2018). WB ; Mouse . Read more (PubMed: 29263200) »
  • Dubovy SR  et al. Expression of hypothalamic neurohormones and their receptors in the human eye. Oncotarget 8:66796-66814 (2017). Read more (PubMed: 28977997) »

See all 12 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (brain hypothalamus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0
Permeabilization
Yes - 0.4% triton x-100 in KPBS
Specification
brain hypothalamus
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 05 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (Hypothalamus)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Hypothalamus
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kevin Zhang

Verified customer

提交于 Nov 17 2017

Application
IHC - Wholemount
Sample
Astatotilapia burtoni Tissue (Brain (CLARITY cleared with Passive clearing))
Specification
Brain (CLARITY cleared with Passive clearing)
Username

Sebastian Alvarado

Verified customer

提交于 May 02 2016

Thank you for contacting us. Both the ab5617 and ab16216 are raised against an immunogen synthetic peptide of HWSYGLRPG corresponding to amino acids 22-31 of GnRH. Comparing this to the corresponding sequence of Chicken GnRH (Swiss-Prot number ...

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Thank you for your enquiry. The concentration of the lot that received in November is approximately 1 mg/ml. Our current batch also has a concentration of approximately 1 mg/ml. Please contact us again if you have any additional questions.

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you dec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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