Anti-GNL3L抗体(ab94862)

概述

  • 产品名称Anti-GNL3L抗体
    参阅全部 GNL3L 一抗
  • 描述
    兔多克隆抗体to GNL3L
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide designed within residues: QAAREQERQK RRTIESYCQD VLRRQEEFEH KEEVLQELNM FPQLDDEATR, corresponding to amino acids 72 - 121 of Human GNL3L (NP_061940).

  • 阳性对照
    • Fetal lung lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab94862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-GNL3L antibody 图像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GNL3L with ab94862 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

  • Anti-GNL3L antibody (ab94862) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Gel concentration 12%

Anti-GNL3L antibody (ab94862)参考文献

ab94862 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human HEK 293T)
Loading amount 20 µg
Specification human HEK 293T
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Pankaj Gupta

Verified customer

提交于 Feb 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"