概述

  • 产品名称Anti-GNAT3抗体
    参阅全部 GNAT3 一抗
  • 描述
    兔多克隆抗体to GNAT3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids 85-113 of Human GNAT3 (NP_001095856.1).

  • 阳性对照
    • Formalin fixed and paraffin embedded Human colon carcinoma A549 cell line lysate

性能

应用

Our Abpromise guarantee covers the use of ab107512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
  • 组织特异性Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa.
  • 序列相似性Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • 翻译后修饰Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane.
  • 细胞定位Cytoplasm. Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GDCA antibody
    • Ggust antibody
    • Gnat 3 antibody
    • GNAT3 antibody
    • GNAT3_HUMAN antibody
    • Gtn antibody
    • Guanine nucleotide binding protein alpha transducing 3 antibody
    • Guanine nucleotide binding protein G(t) subunit alpha 3 antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-3 antibody
    • Gustducin alpha 3 antibody
    • Gustducin alpha 3 chain antibody
    • Gustducin alpha-3 chain antibody
    see all

Anti-GNAT3 antibody 图像

  • Anti-GNAT3 antibody (ab107512) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size : 40 kDa
  • ab107512, at 1/50, staining GNAT3 in formalin fixed and paraffin embedded human colon carcinoma by Immunohistochemistry. Followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-GNAT3 antibody (ab107512)参考文献

ab107512 has not yet been referenced specifically in any publications.

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