概述

  • 产品名称Anti-GNAT2抗体
    参阅全部 GNAT2 一抗
  • 描述
    兔多克隆抗体to GNAT2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GNAT2.

  • 阳性对照
    • This antibody gave a positive signal in both Rat and Mouse Retina tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab97501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).

靶标

  • 功能Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • 组织特异性Retinal rod outer segment.
  • 疾病相关Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination.
  • 序列相似性Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACHM4 antibody
    • Cone type transducin alpha subunit antibody
    • GNAT 2 antibody
    • GNAT C antibody
    • Gnat2 antibody
    • GNAT2_HUMAN antibody
    • GNATC antibody
    • Guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 2 antibody
    • Guanine nucleotide binding protein G t subunit alpha 2 antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-2 antibody
    • Transducin alpha 2 antibody
    • Transducin alpha-2 chain antibody
    • Transducin alpha2 antibody
    • Transducin cone specific alpha polypeptide antibody
    see all

Anti-GNAT2 antibody 图像

  • All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml

    Lane 1 : Mouse Retina Tissue Lysate
    Lane 2 : Rat Retina Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-GNAT2 antibody (ab97501)参考文献

This product has been referenced in:
  • Datta P  et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). WB ; Mouse . Read more (PubMed: 26216965) »
  • Sato K  et al. M-opsin protein degradation is inhibited by MG-132 in Rpe65(-/-) retinal explant culture. Mol Vis 18:1516-25 (2012). WB ; Mouse . Read more (PubMed: 22736942) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Retinal)
Loading amount 10 µg
Specification Retinal
Gel Running Conditions Reduced Denaturing (10% Tris Glycine Gel)
Blocking step 1:1 PBS: Licor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
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Dr. Chris Tracy

Verified customer

提交于 Aug 24 2012

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