概述

  • 产品名称Anti-GNAT1抗体
    参阅全部 GNAT1 一抗
  • 描述
    兔多克隆抗体to GNAT1
  • 经测试应用适用于: IHC-Fr, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized peptide derived from the internal of human Transducin alpha

  • 阳性对照
    • Extracts from COLO cells

性能

应用

Our Abpromise guarantee covers the use of ab74059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/40000.

靶标

  • 功能Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • 组织特异性Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium.
  • 疾病相关Night blindness, congenital stationary, autosomal dominant 3
    Night blindness, congenital stationary, 1C
  • 序列相似性Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • 发展阶段Expressed at approximately postnatal day 7.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSNBAD3 antibody
    • GBT1 antibody
    • GNAT1 antibody
    • GNAT1_HUMAN antibody
    • GNATR antibody
    • guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 1 antibody
    • guanine nucleotide binding protein G(T) alpha 1 subunit antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-1 antibody
    • Rod specific transducin antibody
    • rod-type transducin alpha subunit antibody
    • Transducin alpha-1 chain antibody
    • transducin, rod-specific antibody
    see all

Anti-GNAT1 antibody 图像

  • All lanes : Anti-GNAT1 antibody (ab74059) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 48 kDa,70 kDa,85 kDa. We are unsure as to the identity of these extra bands.

Anti-GNAT1 antibody (ab74059)参考文献

This product has been referenced in:
  • Szabó K  et al. Immunocytochemical analysis of misplaced rhodopsin-positive cells in the developing rodent retina. Cell Tissue Res 356:49-63 (2014). Read more (PubMed: 24496510) »
  • Chucair-Elliott AJ  et al. Leukemia Inhibitory Factor (LIF) coordinates the downregulation of the visual cycle in the retina and retinal pigmented epithelium (RPE). J Biol Chem : (2012). WB ; Mouse . Read more (PubMed: 22645143) »

See all 3 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin, Retina)
Specification Skin, Retina
Fixative Methanol
Permeabilization Yes - 0.4% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Nov 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"