Anti-GMP Synthase抗体(ab87249)

概述

  • 产品名称Anti-GMP Synthase抗体
    参阅全部 GMP Synthase 一抗
  • 描述
    兔多克隆抗体to GMP Synthase
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 252-301 (CTALLNRALN QEQVIAVHID NGFMRKRESQ SVEEALKKLG IQVKVINAAH) of Human GMP Synthase, NP_003866

  • 阳性对照
    • MCF7 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.
  • 通路Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
  • 疾病相关Note=A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.
  • 序列相似性Contains 1 glutamine amidotransferase type-1 domain.
    Contains 1 GMP-binding domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glutamine amidotransferase antibody
    • GMP synthase [glutamine hydrolyzing] antibody
    • GMP synthase [glutamine-hydrolyzing] antibody
    • GMP synthetase antibody
    • Gmps antibody
    • GUAA_HUMAN antibody
    • Guanine monphosphate synthetase antibody
    • Guanosine 5' monophosphate synthase antibody
    • MLL/GMPS fusion protein antibody
    see all

Anti-GMP Synthase antibody 图像

  • Anti-GMP Synthase antibody (ab87249) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa

Anti-GMP Synthase antibody (ab87249)参考文献

ab87249 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Xenopus laevis Cell lysate - whole cell (egg extract (total and chromatin))
Specification egg extract (total and chromatin)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"