Anti-GMP Synthase抗体(ab87249)

概述

  • 产品名称
    Anti-GMP Synthase抗体
    参阅全部 GMP Synthase 一抗
  • 描述
    兔多克隆抗体to GMP Synthase
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 252-301 (CTALLNRALN QEQVIAVHID NGFMRKRESQ SVEEALKKLG IQVKVINAAH) of Human GMP Synthase, NP_003866

  • 阳性对照
    • MCF7 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.
  • 通路
    Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
  • 疾病相关
    Note=A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.
  • 序列相似性
    Contains 1 glutamine amidotransferase type-1 domain.
    Contains 1 GMP-binding domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GATD7 antibody
    • Glutamine amidotransferase antibody
    • GMP synthase [glutamine hydrolyzing] antibody
    • GMP synthase [glutamine-hydrolyzing] antibody
    • GMP synthetase antibody
    • Gmps antibody
    • GUAA_HUMAN antibody
    • Guanine monophosphate synthase antibody
    • Guanine monphosphate synthetase antibody
    • Guanosine 5' monophosphate synthase antibody
    • MLL/GMPS fusion protein antibody
    • Testicular tissue protein Li 82 antibody
    see all

Anti-GMP Synthase antibody 图像

  • Anti-GMP Synthase antibody (ab87249) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa

实验方案

Anti-GMP Synthase antibody (ab87249)参考文献

ab87249 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (egg extract (total and chromatin))
Specification
egg extract (total and chromatin)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 03 2013

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