The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Use a concentration of 4 - 8 µg/ml.
功能Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses.
组织特异性Expressed in peripheral blood lymphocytes and fetal liver. Expressed preferentially in reproductive and/or developmentally important cells, such as testis, placenta, bone marrow and fetal tissues.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling GMEB2 with ab50592 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
Western blot - GMEB2 antibody (ab50592)
Anti-GMEB2 antibody (ab50592) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
ab50592 at 4ug/ml dilution staining human GMEB2 in epithelial cells of renal tubule in human kidney by Immunohistochemistry, Paraffin embedded tissue.ab50592 at 4ug/ml dilution staining human GMEB2 in epithelial cells of renal tubule in human kidney by Immunohistochemistry, Paraffin embedded tissue.
Anti-GMEB2 antibody (ab50592)参考文献
has not yet been referenced specifically in any publications.