Anti-GM130抗体- Drosophila Golgi/Cis-Golgi Marker (ab30637)

概述

  • 产品名称Anti-GM130抗体- Drosophila Golgi/Cis-Golgi Marker
    参阅全部 GM130 一抗
  • 描述
    兔多克隆抗体to GM130 - Drosophila Golgi/Cis-Golgi Marker
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Fruit fly (Drosophila melanogaster) GM130.

    (Peptide available as ab32335.)

  • 阳性对照
    • Drosophila S2 Cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab30637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).

靶标

  • 功能Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
  • 序列相似性Belongs to the GOLGA2 family.
  • 结构域Extended rod-like protein with coiled-coil domains.
  • 细胞定位Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • 别名
    • 130 kDa cis Golgi matrix protein antibody
    • 130 kDa cis-Golgi matrix protein antibody
    • Cis golgi matrix protein GM130 antibody
    • GM130 antibody
    • Gm130 autoantigen antibody
    • GOGA2_HUMAN antibody
    • GOLGA 2 antibody
    • Golga2 antibody
    • Golgi autoantigen antibody
    • Golgi autoantigen golgin subfamily a 2 antibody
    • Golgi matrix protein GM130 antibody
    • Golgin 95 antibody
    • golgin A2 antibody
    • Golgin subfamily a 2 antibody
    • Golgin subfamily A member 2 antibody
    • Golgin-95 antibody
    • MGC20672 antibody
    • SY11 protein antibody
    see all

Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker 图像

  • The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
  • All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/ml

    Lane 1 : Drosophila melanogaster S2 cell extract
    Lane 2 : Drosophila melanogaster S2 cell extract + specific siRNA


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

    This image is courtesy of Sean Munro, MRC Laboratory of Molecular Biology

  • ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
    Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1.
  • All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/ml

    Lane 1 : Schneider L2 whole cell lysate (ab14893)
    Lane 2 : Schneider L2 whole cell lysate (ab14893) with D. melanogaster GM130 peptide (ab32335) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 95 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)参考文献

This product has been referenced in:
  • Mario LC  et al. Egg and fourth instar larvae gut of Aedes aegypti as a source of stem cells. Tissue Cell 48:558-65 (2016). IHC-P, Flow Cyt . Read more (PubMed: 27401144) »
  • Hicks L  et al. Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology. Biol Open 4:1448-61 (2015). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 26459243) »

See all 22 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification total larvae, 4 days post fertilization
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Oct 27 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Fruit fly (Drosophila melanogaster) Cell (Whole Ovary/egg chamber)
Specification Whole Ovary/egg chamber
Permeabilization Yes - 1% Triton-X100 2h 1% BSA
Fixative Paraformaldehyde
Username

Dr. Irina Catrina

Verified customer

提交于 Jun 06 2014

Thank you for your enquiry regarding ab30637 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

After reading through the detailed protocol you k...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for contacting Abcam.

I would like to thank you for your response. If the product has gone off we will gladly replace it. However the procedure for this is that we have the protocol used and troubleshooting steps that you have perf...

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Thank you for contacting us.

I am sorry to hear of the difficulties that you have been experiencing using this product. Would you mind sending me the details of your protocol including the tissue type you are working with and your sample pre...

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Thanks you very much for sending us more details about the experiment. I am sorry I am not sure why the antibody staining appears spotty. This antibody was used in many publications without problem. So perhaps you have received a bad vial of this p...

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Thank you for contacting us and for sending the images. I believe the problem is not due to antibody so I would suggest the following - The antibody is showing consistent results in cells outside the marked area. It looks similar as publicati...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (eye disc)
Specification eye disc
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Apr 28 2008

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