Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) GM130.
(Peptide available as
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22543973
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Belongs to the GOLGA2 family.
Extended rod-like protein with coiled-coil domains.
Golgi apparatus > Golgi stack membrane.
Information by UniProt
130 kDa cis Golgi matrix protein antibody
130 kDa cis-Golgi matrix protein antibody
Cis golgi matrix protein GM130 antibody
Western blot - GM130 antibody (ab32337)
Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
100 kDa (
why is the actual band size different from the predicted?
Additional bands at:
70 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
et al. Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface ?-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). J Biol Chem 289:12896-907 (2014).
Read more (PubMed: 24668805) »
et al. Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. Hum Mol Genet 21:3356-65 (2012).
Read more (PubMed: 22543973) »
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for this product
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