概述

  • 产品名称Anti-GM-CSF抗体
    参阅全部 GM-CSF 一抗
  • 描述
    兔多克隆抗体to GM-CSF
  • 经测试应用适用于: WB, ELISA, Neutralising, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Highly pure (>98%) recombinant mGM-CSF (mouse Granulocyte Macrophage Colony Stimulating Factor)

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

性能

应用

Our Abpromise guarantee covers the use of ab9741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect mGM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mGM-CSF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.6 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 序列相似性Belongs to the GM-CSF family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Colony stimulating factor 2 (granulocyte-macrophage) antibody
    • Colony Stimulating Factor 2 antibody
    • Colony stimulating factor antibody
    • Colony-stimulating factor antibody
    • CSF 2 antibody
    • CSF antibody
    • CSF2 antibody
    • CSF2_HUMAN antibody
    • GM-CSF antibody
    • GMCSF antibody
    • Granulocyte Macrophage Colony Stimulating Factor antibody
    • Granulocyte-macrophage colony-stimulating factor antibody
    • MGC131935 antibody
    • MGC138897 antibody
    • MGI1GM antibody
    • Molgramostin antibody
    • Pluripoietin-a antibody
    • Sargramostim antibody
    see all

Anti-GM-CSF antibody 图像



  • Performed under reducing conditions.

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.



  • Performed under non-reducing conditions.

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.

  • Less than 0.10 ug/ml ab9741 will neutralize 50% of the bioactivity of 1 ng/ml m-GM-CSF.
  • IHC image of GM-CSF staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9741, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GM-CSF antibody (ab9741)参考文献

This product has been referenced in:

See 1 Publication for this product

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Thank you for contacting us.

ab9741 has not been tested in ICC/IF so we do not have any useful information to provide the customer.

Please do not hesitate to contact us if you need any more advice or information.

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The neutralizing and inhibition functions stated on the datasheet of the antibodies ab9741 andab64715 relate to the assays which have been performed to validate their...

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Thank you very much for your inquiry.

The immunogen of ab9741 was the whole GM-CSFprotein of mouse. Mouse GM-CSF only shows 71% homology with rat as a whole protein and therefore I cannot recommend to use this antibody for rat samples.
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Thank you for contacting us with your question.
The GM-CSF protein is expected to run around 16 kDa, however there are 4 known glycosylation sites, each adding around 2 kDa to the protein, so the protein generally runs around 24 kDa on a Western b...

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Unfortunately, we only test against the recombinant murine GM-CSF and do not test tissue samples. In the naturally occurring GM-CSF there are potential glycolisation sites which could cause the protein to migrate higher on a WB. Sorry we coul...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"