兔多克隆抗体to Glypican 5
Synthetic peptide conjugated to KLH, between amino acids 59-88 from the N-terminal region of Human Glypican 5 (NP_004457.1).
Mouse cerebellum tissue lysate, Human brain
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab107493 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 64 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Cell surface proteoglycan that bears heparan sulfate.
In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.
Belongs to the glypican family.
Cell membrane and Secreted > extracellular space.
Information by UniProt
glypican 5 antibody
Glypican 5 precursor antibody
Western blot - Glypican 5 antibody (ab107493)
Anti-Glypican 5 antibody (ab107493) at 1/100 dilution + Mouse cerebellum tissue lysate at 35 µg
Predicted band size: 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glypican 5 antibody (ab107493)
Immunohistochemistry analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107493 at a dilution of 1/50.
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