概述

  • 产品名称Anti-Glypican 4抗体
    参阅全部 Glypican 4 一抗
  • 描述
    兔多克隆抗体to Glypican 4
  • 特异性Specific for a core protein epitope (aa220-233) of human Glypican 4.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    TFAQGLAVAG-DVV

    conjugated to KLH, corresponding to amino acids 220-233 of Human Glypican 4

性能

应用

Our Abpromise guarantee covers the use of ab52509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 62 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
  • 序列相似性Belongs to the glypican family.
  • 细胞定位Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ900E8.1 glypican 4 antibody
    • Glypican proteoglycan 4 antibody
    • Gpc4 antibody
    • GPC4_HUMAN antibody
    • K Glypican antibody
    • K-glypican antibody
    • Kglypican antibody
    • OTTHUMP00000024052 antibody
    • Secreted glypican 4 antibody
    • Secreted glypican-4 antibody
    • UNQ474/PRO937 antibody
    see all

Anti-Glypican 4 antibody 图像

  • IHC image of ab52509 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52509, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glypican 4 antibody (ab52509)参考文献

This product has been referenced in:
  • Iacobas S  et al. The connexin43-dependent transcriptome during brain development: Importance of genetic background. Brain Res : (2012). Read more (PubMed: 22771707) »

See 1 Publication for this product

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