Anti-Glypican 4抗体(ab118911)

概述

  • 产品名称
    Anti-Glypican 4抗体
    参阅全部 Glypican 4 一抗
  • 描述
    兔多克隆抗体to Glypican 4
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to the C-terminal domain of Human Glypican 4.

  • 阳性对照
    • Human Liver and Human Pancreas tissues.

性能

应用

Our Abpromise guarantee covers the use of ab118911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 15 - 20 µg/ml.

靶标

图片

  • ab118911 staining Glypican in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Blue - nuclei (DAPI)

    See Abreview

  • ab118911, at 20 µg/ml, staining Glypican 4 in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab118911, at 20 µg/ml, staining Glypican 4 in Formalin-fixed, Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.

文献

ab118911 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Human Tissue sections (human pancreas)
Specification
human pancreas
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 17 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Cow Tissue sections (bovine kidney)
Specification
bovine kidney
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Jan 23 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册