概述

  • 产品名称
    Anti-Glypican 1/ GPC1抗体
    参阅全部 Glypican 1/ GPC1 一抗
  • 描述
    兔多克隆抗体to Glypican 1/ GPC1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) corresponding to a C-terminal sequence.

性能

应用

Our Abpromise guarantee covers the use of ab55971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent dilution.

靶标

图片

  • Anti-Glypican 1/ GPC1 antibody (ab55971) at 1/2000 dilution + lysate prepared from ScN2a cells.

    Secondary
    peroxidase-conjugated secondary

    Developed using the ECL technique.

    Predicted band size: 62 kDa



    Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.

实验方案

文献

This product has been referenced in:
  • Cave C  et al. GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord. Mol Neurodegener 12:8 (2017). Read more (PubMed: 28103900) »
  • Sharma P  et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun 6:8391 (2015). WB . Read more (PubMed: 26403541) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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