概述

  • 产品名称Anti-GLYCTK抗体
    参阅全部 GLYCTK 一抗
  • 描述
    小鼠单克隆抗体to GLYCTK
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cynomolgus Monkey
  • 免疫原

    Recombinant fragment: VLSAAMQGDV KSMAQFYGLL AHVARTRLTP SMAGASVEED AQLHELAAEL QIPDLQLEEA LETMAWGRGP VCLLAGGEPT VQLQGSGRGG RNQELALRV, corresponding to amino acids 332-430 of human GLYCTK (NP_660305) with a 26 kDa tag

  • 阳性对照
    • NIH/3T3 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab77727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 组织特异性Widely expressed.
  • 疾病相关Defects in GLYCTK are the cause of D-glyceric aciduria (D-GA) [MIM:220120]. D-GA is a rare metabolic disease characterized by chronic metabolic acidosis and a highly variable clinical phenotype. Clinical features range from an encephalopathic presentation with seizures, microcephaly, severe mental retardation and early death, to milder manifestations with only speech delay or even normal development.
  • 序列相似性Belongs to the glycerate kinase type-2 family.
  • 细胞定位Cytoplasm and Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CG9886 like antibody
    • GLCTK_HUMAN antibody
    • Glycerate kinase antibody
    • Glyctk antibody
    • GLYCTK1 antibody
    • HBeAg binding protein 2 antibody
    • HBeAg binding protein 4 antibody
    • HBeAg-binding protein 4 antibody
    • HBeAgBP4A antibody
    • HBEBP2 antibody
    • HBEBP4 antibody
    see all

Anti-GLYCTK antibody 图像

  • Anti-GLYCTK antibody (ab77727) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 55 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-GLYCTK antibody (ab77727) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 55 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

Anti-GLYCTK antibody (ab77727)参考文献

ab77727 has not yet been referenced specifically in any publications.

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