RabMAb

Anti-Glycoprotein hormones (alpha 5)抗体[EP3373] (ab92738)

概述

  • 产品名称Anti-Glycoprotein hormones (alpha 5)抗体[EP3373]
  • 描述
    兔单克隆抗体[EP3373] to Glycoprotein hormones (alpha 5)
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glycoprotein hormones (alpha 5) aa 1-100.
    Database link: P01215

  • 阳性对照
    • JAR cell lysates treated with Hydroxyurea; Human placenta tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab92738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/2000.

For unpurified, use 1/100 - 1/250.

靶标

  • 相关性The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The alpha subunit belongs to the glycoprotein hormones alpha chain family.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • Anterior pituitary glycoprotein hormones common subunit alpha antibody
    • CG alpha antibody
    • CGA antibody
    • Choriogonadotropin alpha chain antibody
    • Chorionic gonadotrophin subunit alpha antibody
    • Chorionic gonadotropin, alpha polypeptide antibody
    • Follicle stimulating hormone alpha chain antibody
    • Follicle stimulating hormone alpha subunit antibody
    • Follitropin alpha chain antibody
    • FSH alpha antibody
    • Glycoprotein hormones alpha chain antibody
    • Glycoprotein hormones, alpha polypeptide antibody
    • LSH alpha antibody
    • Luteinizing hormone alpha chain antibody
    • Lutropin alpha chain antibody
    • Pituitary hormone antibody
    • Thyroid stimulating hormone alpha chain antibody
    • Thyrotropin alpha chain antibody
    • TSH alpha antibody
    see all

Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] 图像

  • Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738) at 1/5000 dilution (purified) + human placenta at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738) at 1/1000 dilution (purified) + Hydroxyurea treated JAR whole cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human placenta with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human pancreas with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • All lanes : Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738) at 1/500 dilution (unpurified)

    Lane 1 : JAR cell lysates, treated with Hydroxyurea
    Lane 2 : JAR cell lysates, untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • Unpurified ab92738 showing negative staining in Normal breast tissue.

  • Unpurified ab92738 showing negative staining in Normal kidney tissue.

Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738)参考文献

ab92738 has not yet been referenced specifically in any publications.

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