Anti-Glycophorin A抗体[JC159] (ab9520)


  • 产品名称Anti-Glycophorin A抗体[JC159]
    参阅全部 Glycophorin A 一抗
  • 描述
    小鼠单克隆抗体[JC159] to Glycophorin A
  • 特异性This antibody is specific to erythroid cells at various stages of differentiation from the erythroblast to the mature red cell.
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Formalin fixed membrane from a Hairy cell leukemia.

  • 阳性对照
    • Tonsil



Our Abpromise guarantee covers the use of ab9520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 功能Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • 序列相似性Belongs to the glycophorin A family.
  • 翻译后修饰The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • 细胞定位Cell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI853584 antibody
    • Blood group--MN locus antibody
    • CD_antigen=CD235a antibody
    • CD235a antibody
    • GLPA_HUMAN antibody
    • Glycophorin A (MNS blood group) antibody
    • Glycophorin A antibody
    • Glycophorin A, included antibody
    • Glycophorin-A antibody
    • GlycophorinA antibody
    • GPA antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPA, included antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS-2 antibody
    • PAS2 antibody
    • Sialoglycoprotein alpha antibody
    see all

Anti-Glycophorin A antibody [JC159] 图像

  • Ab9520 staining Human lung. Staining is localised to red blood cells in lung tissue.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Glycophorin A with ab9520.

Anti-Glycophorin A antibody [JC159] (ab9520)参考文献

This product has been referenced in:
  • Estarás C  et al. Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Development 139:2681-91 (2012). Read more (PubMed: 22782721) »

See 1 Publication for this product

Product Wall

Thank you for your reply. In general, most antibodies have some degree of non-specific binding. For flow cytometry, I can recommend BSA or FCS recommend in staining and wash buffers. Some samples (e.g. cells with Fc receptors li...

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Thank you for your inquiry. As my colleague mentioned in the previous email, this antibody is not tested and guaranteed for flow cytometry. Unfortunately, we do not have any information about its suitability for flow cytometry. Suitable ...

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Thank you for your enquiry and your interest in our products. Generally, if the antibody is provided as ascitic fluid and not affinity purified product; we do not determine the IgG concentration. Ab9520 is an exemption, the protein concentration ...

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