概述

  • 产品名称Anti-Glycophorin A抗体[BRIC 256]
    参阅全部 Glycophorin A 一抗
  • 描述
    小鼠单克隆抗体[BRIC 256] to Glycophorin A
  • 特异性ab35760 recognises Glycophorin A which is widely found on all erythroid cells and tissues. There are approx 500,000 GPA molecules per RBC.
  • 经测试应用适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native Glycophorin Aprotein (Human).

  • 表位Epitope around aa 49-52 on the extracellular domain of Glycophorin A(approx 70 aa). Possibly trypsin resistant epitope.

性能

应用

Our Abpromise guarantee covers the use of ab35760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. PubMed: 24043627
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.

靶标

  • 功能Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • 序列相似性Belongs to the glycophorin A family.
  • 翻译后修饰The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • 细胞定位Cell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI853584 antibody
    • Blood group--MN locus antibody
    • CD_antigen=CD235a antibody
    • CD235a antibody
    • GLPA_HUMAN antibody
    • Glycophorin A (MNS blood group) antibody
    • Glycophorin A antibody
    • Glycophorin A, included antibody
    • Glycophorin-A antibody
    • GlycophorinA antibody
    • GPA antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPA, included antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS-2 antibody
    • PAS2 antibody
    • Sialoglycoprotein alpha antibody
    see all

Anti-Glycophorin A antibody [BRIC 256] (ab35760)参考文献

This product has been referenced in:
  • Wanaguru M  et al. Biochemical analysis of the Plasmodium falciparum erythrocyte-binding antigen-175 (EBA175)-glycophorin-A interaction: implications for vaccine design. J Biol Chem 288:32106-17 (2013). ELISA ; Human . Read more (PubMed: 24043627) »
  • Khattab A & Meri S Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface. Malar J 10:58 (2011). WB ; Human . Read more (PubMed: 21401937) »

See all 3 Publications for this product

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