Anti-Glycogen synthase 2抗体(ab83550)

概述

  • 产品名称Anti-Glycogen synthase 2抗体
  • 描述
    兔多克隆抗体to Glycogen synthase 2
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: TLSRAFPDKF HVELTSPPTT EGFKYPRPSS VPPSPSGSQA SSPQSSDVED , corresponding to a region within internal sequence amino acids 612 - 661 of Human Glycogen synthase 2 (NP_068776)

  • 阳性对照
    • Fetal muscle lysate

性能

应用

Our Abpromise guarantee covers the use of ab83550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • 通路Glycan biosynthesis; glycogen biosynthesis.
  • 疾病相关Defects in GYS2 are the cause of glycogen storage disease type 0 (GSD0) [MIM:240600]; A metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood, high blood ketones and low alanine and lactate concentrations. Although feeding relieves symptoms, it often results in postprandial hyperglycemia and hyperlactatemia.
  • 序列相似性Belongs to the glycosyltransferase 3 family.
  • 翻译后修饰Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.4.1.11 antibody
    • Glycogen [starch] synthase antibody
    • Glycogen starch synthase liver antibody
    • Glycogen synthase 2 liver antibody
    • Gys2 antibody
    • GYS2_HUMAN antibody
    • liver antibody
    see all

Anti-Glycogen synthase 2 antibody 图像

  • Anti-Glycogen synthase 2 antibody (ab83550) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
  • IHC image of ab83550 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83550, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glycogen synthase 2 antibody (ab83550)参考文献

ab83550 has not yet been referenced specifically in any publications.

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