重组
RabMAb

Anti-Glycogen synthase 1 (phospho S641)抗体[EP852Y] (ab81230)

概述

  • 产品名称
    Anti-Glycogen synthase 1 (phospho S641)抗体[EP852Y]
    参阅全部 Glycogen synthase 1 一抗
  • 描述
    兔单克隆抗体[EP852Y] to Glycogen synthase 1 (phospho S641)
  • 特异性
    Detects Glycogen synthase 1 only when phosphorylated on Serine 641.
  • 经测试应用
    适用于: Dot blot, WB, IHC-P, IPmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    corresponding to Human Glycogen synthase 1.

  • 阳性对照
    • WB: Mouse muscle and Mouse muscle treated with AP lysates; HeLa and HeLa treated with insulin at 20 ug/mL for 15 minutes whole cell lysates. IHC: Human liver tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Glycogen synthase 1 (phospho S641) antibody (Biotin) [EP852Y] (ab201509)

性能

应用

Our Abpromise guarantee covers the use of ab81230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot 1/1000.
WB 1/10000 - 1/2000. Predicted molecular weight: 85 kDa.
IHC-P 1/100 - 1/250.
IP 1/60.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能
      Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
    • 通路
      Glycan biosynthesis; glycogen biosynthesis.
    • 疾病相关
      Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
    • 序列相似性
      Belongs to the glycosyltransferase 3 family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Glycogen [starch] synthase antibody
      • Glycogen synthase 1 (muscle) antibody
      • Glycogen synthase 1 antibody
      • GSY antibody
      • GYS antibody
      • Gys1 antibody
      • GYS1_HUMAN antibody
      • muscle antibody
      see all

    Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] 图像

    • All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] (ab81230) at 1/2000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with insulin at 20 ug/mL for 15 minutes. Whole cell lysates
      Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.
      Lane 3 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000

      Predicted band size : 85 kDa
      Observed band size : 85 kDa


      Exposure time : 1 second

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    • All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] (ab81230) at 1/10000 dilution

      Lane 1 : Mouse muscle lysate, untreated
      Lane 2 : Mouse muscle lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 85 kDa
      Observed band size : 85 kDa
    • Dot blot analysis of glycogen synthase 1 (phospho S641) phospho peptide (Lane 1) and glycogen synthase 1 non-phospho peptide (Lane 2) labeling synthase 1 (phospho S641) with ab81230 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230.

    Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] (ab81230)参考文献

    This product has been referenced in:
    • Zhang JS  et al. Differential activity of GSK-3 isoforms regulates NF-?B and TRAIL- or TNFa induced apoptosis in pancreatic cancer cells. Cell Death Dis 5:e1142 (2014). WB . Read more (PubMed: 24675460) »
    • Reichelt ME  et al. Myocardial glycophagy - A specific glycogen handling response to metabolic stress is accentuated in the female heart. J Mol Cell Cardiol 65C:67-75 (2013). Mouse . Read more (PubMed: 24080183) »

    See all 4 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel)
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 28 2013

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Username

    Dr. Karine Thibault

    Verified customer

    提交于 Nov 17 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (gel 10% SDS-PAGE)
    Sample
    Mouse Tissue lysate - whole (substantia nigra)
    Specification
    substantia nigra
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Karine Thibault

    Verified customer

    提交于 Mar 05 2015

    I apologize for the delays due to retesting of this antibody.

    Lab has confirmed observing a band at 85kDa in human, rat and mouse muscle lysates. They have provided an image which clearly show the antibody is detecting a correct band at 85kDa...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册