Anti-Glycogen synthase 1 (phospho S640)抗体(ab2479)

概述

  • 产品名称
    Anti-Glycogen synthase 1 (phospho S640)抗体
    参阅全部 Glycogen synthase 1 一抗
  • 描述
    兔多克隆抗体to Glycogen synthase 1 (phospho S640)
  • 特异性
    This phospho specific polyclonal antibody is specific for phosphorylated Ser 640 of human muscle glycogen synthase (GYS1). Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Human Muscle Glycogen Synthase (GYS1) phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - AQGYRYPRPA(pS)VP (aa 631-642).

  • 常规说明


    Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.

性能

应用

Our Abpromise guarantee covers the use of ab2479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000.
Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.

靶标

  • 功能
    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • 通路
    Glycan biosynthesis; glycogen biosynthesis.
  • 疾病相关
    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
  • 序列相似性
    Belongs to the glycosyltransferase 3 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all

图片

  • Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

  • All lanes : Anti-Glycogen synthase 1 (phospho S640) antibody (ab2479) at 1/1000 dilution

    Lane 1 : mock treated mouse cardiac myocyte lysate at 12 µl
    Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
    Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
    Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
    Lane 5 : mock treated mouse cardiac myocyte lysate at 12 µl
    Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
    Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
    Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

    Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot.  Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. 

    Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.

    A 4-20% Criterion gel for SDS-PAGE was used.

文献

This product has been referenced in:
  • Eckle T  et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). Read more (PubMed: 22504483) »
  • Ambacher KK  et al. The JNK- and AKT/GSK3ß- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS One 7:e46885 (2012). Read more (PubMed: 23056511) »

See all 4 Publications for this product

客户评价及客户问答

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). This antibody has been tested for Western blot application, so it should work nicely. There is no publ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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