Anti-Glycine Receptor alpha 1 + alpha 2抗体(ab23809)

概述

  • 产品名称Anti-Glycine Receptor alpha 1 + alpha 2抗体
  • 描述
    兔多克隆抗体to Glycine Receptor alpha 1 + alpha 2
  • 特异性This product recognises an epitope within the N-terminal region of glycine receptor alpha 1 and alpha 2 subunits.
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (N-terminal) (Rat).

  • 阳性对照
    • Rat brain lysate, rat spinal cord lysate.
  • 常规说明For long term storage the addition of 0.09% sodium azide is recommended.

性能

应用

Our Abpromise guarantee covers the use of ab23809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/1000.
WB 1/1000. Detects a band of approximately 48 kDa.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/1000 for overnight incubation on rat cells (see Abreview submitted by Johana Trojanova).
IHC-FoFr Use at an assay dependent concentration. PubMed: 21215254

靶标

Anti-Glycine Receptor alpha 1 + alpha 2 antibody 图像

  •     ab23809 at a 1/1000 dilution staining rat brain stem cells(medial nucleus of trapezoid body) by ICC/IF. The cells were fixed with paraformaldehyde and incubated with the antibody for 18 hours. An Alexa Fluor ® 488 donkey anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab23809 staining Glycin Receptor alpha1 + alpha 2 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/400 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

Anti-Glycine Receptor alpha 1 + alpha 2 antibody (ab23809)参考文献

This product has been referenced in:
  • Ren M  et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016). ICC/IF ; Rat . Read more (PubMed: 27112667) »
  • Maskey D  et al. Alteration of glycine receptor immunoreactivity in the auditory brainstem of mice following three months of exposure to radiofrequency radiation at SAR 4.0 W/kg. Int J Mol Med 34:409-19 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24866721) »

See all 8 Publications for this product

Product Wall

Thank you for providing that information.
We would recommend storing the antibody at -20 degrees C, however, I was trying to identify if any other factor which could be contributing to the loss in activity. From the information the customer has pr...

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Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
In order to und...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse retina sections)
Specification mouse retina sections
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Kevin Jin

Verified customer

提交于 Nov 19 2010

I am sorry to hear your customer is having a problem with ab23809. Thank you for providing the customer's protocol as well as an image, this helped me understand the problem very easily. The image shows positive staining with some sections ("pr...

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Thank you for your enquiry. Your approach is very much akin to the approach made for frozen sections. I would therefore recommend that you perform this standard approach omitting antigen retrieval. If you decide to go ahead and purchase this pr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Brain Stem, Medial Nucleus of Trapezoid Body,)
Specification Brain Stem, Medial Nucleus of Trapezoid Body,
Fixative Paraformaldehyde
Blocking step Other as blocking agent for 16 hour(s) and 1 minute(s) · Concentration: 5%
Username

Ms. Johana Trojanova

Verified customer

提交于 Sep 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"