重组Anti-Glycerol kinase抗体[EPR6567] (ab126599)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6567] to Glycerol kinase
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Glycerol kinase抗体[EPR6567]
参阅全部 Glycerol kinase 一抗 -
描述
兔单克隆抗体[EPR6567] to Glycerol kinase -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Glycerol kinase aa 450-550. The exact sequence is proprietary.
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阳性对照
- WB: Mouse adipose tissue lysate. Fetal liver, HEK-293T, Jurkat and HepG2 whole cell lysate (ab7900). Flow Cyt (intra): Permeabilized HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 4.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6567 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126599于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa). |
靶标
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功能
Key enzyme in the regulation of glycerol uptake and metabolism. -
组织特异性
Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver. -
通路
Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. -
疾病相关
Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only. -
序列相似性
Belongs to the FGGY kinase family. -
细胞定位
Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2710 Human
- Entrez Gene: 14933 Mouse
- Entrez Gene: 79223 Rat
- Omim: 300474 Human
- SwissProt: P32189 Human
- SwissProt: Q64516 Mouse
- SwissProt: Q63060 Rat
- Unigene: 1466 Human
see all -
别名
- ATP glycerol 3 phosphotransferase antibody
- ATP:glycerol 3 phosphotransferase antibody
- ATP:glycerol 3-phosphotransferase antibody
see all
图片
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All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : GK knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDaLanes 1-4: Merged signal (red and green). Green - ab126599 observed at 61 kDa. Red - loading control ab8245 observed at 36 kDa.
ab126599 Anti-Glycerol kinase antibody [EPR6567] was shown to specifically react with Glycerol kinase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab267328 (knockout cell lysate ab257966) was used. Wild-type and Glycerol kinase knockout samples were subjected to SDS-PAGE. ab126599 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution
All lanes : Mouse adipose tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated Donkey anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
ab126599, at 1/100 dilution, staining Glycerol kinase in permeabilized HepG2 cells by ImmunoFluorescence (red). A rabbit IgG is used as a negative control (green).
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab126599 被引用在 8 文献中.
- Yu P et al. Cardiomyocyte-restricted high-mobility group box 1 (HMGB1) deletion leads to small heart and glycolipid metabolic disorder through GR/PGC-1a signalling. Cell Death Discov 6:106 (2020). PubMed: 33101708
- Iena FM et al. Sex-Specific Effect of High-Fat Diet on Glycerol Metabolism in Murine Adipose Tissue and Liver. Front Endocrinol (Lausanne) 11:577650 (2020). PubMed: 33193093
- Miao L et al. Glycerol kinase interacts with nuclear receptor NR4A1 and regulates glucose metabolism in the liver. FASEB J 33:6736-6747 (2019). PubMed: 30821173
- Nikolaou KC et al. The RNA-Binding Protein A1CF Regulates Hepatic Fructose and Glycerol Metabolism via Alternative RNA Splicing. Cell Rep 29:283-300.e8 (2019). PubMed: 31597092
- Kang M et al. Improved systemic metabolism and adipocyte biology in miR-150 knockout mice. Metabolism N/A:N/A (2018). WB ; Mouse . PubMed: 29352962
- Lasar D et al. Peroxisome Proliferator Activated Receptor Gamma Controls Mature Brown Adipocyte Inducibility through Glycerol Kinase. Cell Rep 22:760-773 (2018). WB . PubMed: 29346772
- Schreiber R et al. Cold-Induced Thermogenesis Depends on ATGL-Mediated Lipolysis in Cardiac Muscle, but Not Brown Adipose Tissue. Cell Metab 26:753-763.e7 (2017). Mouse . PubMed: 28988821
- Mottillo EP et al. Coupling of lipolysis and de novo lipogenesis in brown, beige, and white adipose tissues during chronic ß3-adrenergic receptor activation. J Lipid Res 55:2276-86 (2014). PubMed: 25193997