Anti-Glycerol kinase抗体(ab88505)

概述

  • 产品名称Anti-Glycerol kinase抗体
    参阅全部 Glycerol kinase 一抗
  • 描述
    小鼠多克隆抗体to Glycerol kinase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-524 of Human Glycerol kinase (NP_000158.1)

  • 阳性对照
    • human placenta lysate, transfected 293T cell line, human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab88505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Key enzyme in the regulation of glycerol uptake and metabolism.
    • 组织特异性Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
    • 通路Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
    • 疾病相关Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
    • 序列相似性Belongs to the FGGY kinase family.
    • 细胞定位Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3-phosphotransferase antibody
      • D930012N15Rik antibody
      • GK antibody
      • GK1 antibody
      • GKD antibody
      • GLPK_HUMAN antibody
      • Glycerokinase antibody
      • Glycerol kinase antibody
      • OTTHUMP00000023108 antibody
      • OTTHUMP00000023109 antibody
      • OTTHUMP00000215321 antibody
      see all

    Anti-Glycerol kinase antibody 图像

    • Anti-Glycerol kinase antibody (ab88505) at 5 µg/ml + human placenta lysate at 50 µg

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 57 kDa
    • All lanes : Anti-Glycerol kinase antibody (ab88505) at 5 µg/ml

      Lane 1 : transfected 293T cell line
      Lane 2 : non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 57 kDa
    • Immunohistochemical analysis of Glycerol kinase expression in formalin-fixed paraffin-embedded human kidney using 3ug ab88505.

    Anti-Glycerol kinase antibody (ab88505)参考文献

    ab88505 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
    Antigen retrieval step Heat mediated
    Sample Mouse Tissue sections (Adipose tissue)
    Specification Adipose tissue
    Permeabilization Yes - PBS 0.5% TritonX-100
    Fixative Paraformaldehyde
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    提交于 Dec 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"