Anti-Glycerol kinase抗体(ab193521)
Key features and details
- Rabbit polyclonal to Glycerol kinase
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
概述
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产品名称
Anti-Glycerol kinase抗体
参阅全部 Glycerol kinase 一抗 -
描述
兔多克隆抗体to Glycerol kinase -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Recombinant full length protein corresponding to Escherichia coli Glycerol kinase aa 1 to the C-terminus.
Database link: P0A6F3 -
阳性对照
- WB: DH5a whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
>95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193521于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/5000. Predicted molecular weight: 57 kDa.
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说明 |
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WB
1/500 - 1/5000. Predicted molecular weight: 57 kDa. |
靶标
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功能
Key enzyme in the regulation of glycerol uptake and metabolism. -
组织特异性
Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver. -
通路
Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. -
疾病相关
Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only. -
序列相似性
Belongs to the FGGY kinase family. -
细胞定位
Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 12934168 Escherichia coli
- SwissProt: P0A6F3 Escherichia coli
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别名
- ATP glycerol 3 phosphotransferase antibody
- ATP:glycerol 3 phosphotransferase antibody
- ATP:glycerol 3-phosphotransferase antibody
see all
图片
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Anti-Glycerol kinase antibody (ab193521) at 2 µg/ml + DH5a whole cell lysate
Secondary
Polyclonal goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Glycerol kinase antibody (ab193521) at 3 µg/ml
Lane 1 : Rosseta bacteria lysate at 80 µg
Lane 2 : Rosseta bacteria lysate at 40 µg
Lane 3 : Rosseta bacteria lysate at 20 µg
Lane 4 : Rosseta bacteria lysate at 10 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab193521 尚未被引用在任何文献中。