Anti-Glycerol 3 Phosphate Dehydrogenase抗体(ab87286)

概述

  • 产品名称Anti-Glycerol 3 Phosphate Dehydrogenase抗体
    参阅全部 Glycerol 3 Phosphate Dehydrogenase 一抗
  • 描述
    兔多克隆抗体to Glycerol 3 Phosphate Dehydrogenase
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human, Schizosaccharomyces pombe
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 252-301 (FLESCGVADL ITTCYGGRNR KVAEAFARTG KSIEQLEKEL LNGQKLQGPE) of human Glycerol 3 Phosphate Dehydrogenase (NP_005267).

  • 阳性对照
    • Transfected 293T cell lysate

应用

Our Abpromise guarantee covers the use of ab87286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Glycerol 3 Phosphate Dehydrogenase antibody 图像

  • Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab87286) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa

Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab87286)参考文献

ab87286 has not yet been referenced specifically in any publications.

Product Wall

We have customer feedback in the form of an Abreview that suggests this antibody will work in Schizosaccharomyces pombe. See the review here: http://www.abcam.com/ab87286.html#ab87286.html?productWallTab=Abreviews&_suid=1373039857305015358835516314567<...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Schizosaccharomyces pombe Cell lysate - whole cell (Wild type, Gpd1 deletion or Gpd2 deletion strains)
Loading amount 10 µg
Specification Wild type, Gpd1 deletion or Gpd2 deletion strains
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

提交于 Jun 19 2012

That's great news! Thank you very much for your valuable feedback.

Just go to the online datasheet for ab87286 and click "Submit an Abreview" at the top of the page below the product name.

http://www.abcam.com/Glycerol-3-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"