重组
RabMAb

Anti-Glutathione Synthetase抗体[EPR6562] (ab124811)

概述

  • 产品名称
    Anti-Glutathione Synthetase抗体[EPR6562]
    参阅全部 Glutathione Synthetase 一抗
  • 描述
    兔单克隆抗体[EPR6562] to Glutathione Synthetase
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
    不适用于: ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Glutathione Synthetase.

  • 阳性对照
    • 293T, HeLa, SW480 and HT 1080 cell lysates; Human kidney tissue.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for ICC/IF or IP.
  • 靶标

    • 通路
      Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
    • 疾病相关
      Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
      Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
    • 序列相似性
      Belongs to the eukaryotic GSH synthase family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • epididymis secretory sperm binding protein Li 64p antibody
      • epididymis secretory sperm binding protein Li 88n antibody
      • Glutathione synthase antibody
      • Glutathione synthetase antibody
      • GSH S antibody
      • GSH synthetase antibody
      • GSH-S antibody
      • GSHB_HUMAN antibody
      • GSHS antibody
      • GSS antibody
      • HEL-S-64p antibody
      • HEL-S-88n antibody
      • MGC14098 antibody
      • OTTHUMP00000030711 antibody
      see all

    图片

    • All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HT 1080 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    • ab124811, at 1/50 dilution staining Glutathione Synthetase in paraffin-embedded Human kidney tissue, by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    ab124811 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (B16 tumor lysate)
    Gel Running Conditions
    Reduced Denaturing (4-20% TG gels)
    Loading amount
    50 µg
    Specification
    B16 tumor lysate
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 10 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin)
    Loading amount
    50 µg
    Specification
    THP1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 21 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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