重组
RabMAb

Anti-Glutathione Synthetase抗体[EPR6562] (ab124811)

概述

  • 产品名称
    Anti-Glutathione Synthetase抗体[EPR6562]
    参阅全部 Glutathione Synthetase 一抗
  • 描述
    兔单克隆抗体[EPR6562] to Glutathione Synthetase
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
    不适用于: ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Glutathione Synthetase. The exact sequence is proprietary.

  • 阳性对照
    • 293T, HeLa, SW480 and HT 1080 cell lysates; Human kidney tissue.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for ICC/IF or IP.
  • 靶标

    • 通路
      Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
    • 疾病相关
      Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
      Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
    • 序列相似性
      Belongs to the eukaryotic GSH synthase family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • epididymis secretory sperm binding protein Li 64p antibody
      • epididymis secretory sperm binding protein Li 88n antibody
      • Glutathione synthase antibody
      • Glutathione synthetase antibody
      • GSH S antibody
      • GSH synthetase antibody
      • GSH-S antibody
      • GSHB_HUMAN antibody
      • GSHS antibody
      • GSS antibody
      • HEL-S-64p antibody
      • HEL-S-88n antibody
      • MGC14098 antibody
      • OTTHUMP00000030711 antibody
      see all

    图片

    • All lanes : Anti-Glutathione Synthetase antibody [EPR6562] (ab124811) at 1/10000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HT 1080 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    • ab124811, at 1/50 dilution staining Glutathione Synthetase in paraffin-embedded Human kidney tissue, by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    ab124811 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U937 cells)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    U937 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 24 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (B16 cells)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    B16 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 24 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (B16 tumor lysate)
    Gel Running Conditions
    Reduced Denaturing (4-20% TG gels)
    Loading amount
    50 µg
    Specification
    B16 tumor lysate
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 10 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin)
    Loading amount
    50 µg
    Specification
    THP1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 21 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册