The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
功能Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
序列相似性Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
结构域The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
Anti-Glutathione S Transferase alpha 1 antibody 图像
Western blot - Anti-Glutathione S Transferase alpha 1 antibody (ab55129)
Western blot against tagged recombinant protein immunogen using ab55129 Glutathione S Transferase alpha antibody at 1ug/ml. Predicted band size of immunogen is 51 kDa
Anti-Glutathione S Transferase alpha 1 antibody (ab55129)参考文献
This product has been referenced in:
Hardwick RN et al. Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease. Drug Metab Dispos38:2293-301 (2010).
Read more (PubMed: 20805291) »